69. INVASIVENESS OF ESCHERICHIA COLI IN HEp-2 AND HUMAN COLONIC EPITHELIAL CELLS
نویسندگان
چکیده
منابع مشابه
Enhancement of invasiveness of Yersinia enterocolitica and Escherichia coli in HEp-2 cells by centrifugation.
Centrifugation enhanced the infectivity of invasive Escherichia coli and Yersinia enterocolitica for HEp-2 cells. Noninvasive bacteria were not endocytosed after centrifugation. The centrifugation procedure may increase the sensitivity of testing for bacterial invasiveness in cell culture without causing false-positive results.
متن کاملRole of plasmid-encoded adherence factors in adhesion of enteropathogenic Escherichia coli to HEp-2 cells.
Plasmid-encoded adherence factors have been shown to be important for the full expression of enteropathogenic Escherichia coli (EPEC) pathogenicity and for EPEC adhesion to cultured HEp-2 cells. EPEC strain E2348 (O127) shows localized HEp-2 cell adhesion and possesses a 60-megadalton plasmid, pMAR2. When E2348 is cured of pMAR2 it loses the ability to adhere to HEp-2 cells, while nonadherent E...
متن کاملInhibition of enteropathogenic Escherichia coli (EPEC) adherence to HEp-2 cells by bovine colostrum and milk.
BACKGROUND enteropathogenic Escherichia coli (EPEC) is the main etiological agent of infantile diarrhea in Brazil and other developing countries. Human milk IgA protects newborn intestinal mucosa by inhibiting bacterial adhesion to epithelial cells and this effect is shown by in vitro assays of EPEC adhesion to HEp-2 cultured cells. Bovine milk, if effective in promoting this protection, could ...
متن کاملSilencing Bmi1 inhibits the proliferation and invasiveness in CD133+ cancer stem cells of Hep-2 cells
It has been shown that over-expression of B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi1) play a key role in maintaining the stem-like properties in CD133+ Hep-2 cells. This study was aimed at investigating the effects of silencing Bmi11 on CD133+ Hep-2 cells. Bmi1 silencing was achieved by transfection with the vectors expressing small hairpin RNA (shRNA) versus Bmi1. Cel...
متن کاملAggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes.
Strains of enteroaggregative Escherichia coli (EAggEC) have been implicated in several studies as important agents of persistent diarrhea among infants in the developing world. We have previously shown that the aggregative adherence (AA) property of EAggEC is associated with the presence of a 60-MDa plasmid which confers AA when introduced into E. coli HB101. Here, we report the cloning of the ...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1987
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-198707000-00090